| Genes |
Forward primer (F) Reverse primer (R) |
Gene Bank accession no. |
Size of PCR product (bp) |
Annealing temperature (°C) |
| NANOG |
F: CCTCTTAAATTTTTTCCTCCTCTTC
R: AAGTGGGTTGTTTGCCTTTG |
NM_024865 |
271 |
54 |
Oct-4 (Pou5f1; POU class
5 homeobox 1) |
F: TGCCGTGAAACTGAAGAAG
R: TTTCTGCAGAGCTTTGATGTTC |
NM_203289 |
72 |
53 |
| Osteonectin |
F: TCTTCCCTGTACACTGGCAGTTC
R: AGCTCGGTGTGGGAGAGGTA |
NM_003118 |
73 |
55 |
Runx2 (runt-related
transcription factor 2) |
F: CAGACCAGCAGCACTCCATA
R: CAGCGTCAACACCATCATTC |
NM_004348 |
178 |
57 |
| b-actin |
F: CTACAATGAGCTGCGTGTGG
R: CGGTGAGGATCTTCATGAGG |
NM_001101 |
314 |
60 |
