| Cells were treated with different concentrations (0, 0.1, 0.5 and 1mM) of H2O2
for 6 h. IGF-1 and Nrf2 mRNA expression was measured using real-time PCR
(A, C). IGF-1 and Nrf2 proteins were measured using immunoprecipitation
and Western blotting (B, D). The mean intensity was measured using
densitometry. The mRNA and protein levels decreased treatment with 1 mM
H2O2 (A, B, C, and D). The mean intensity was measured using densitometry.
(n=8). Statistical analysis; *p<0.05 vs. negative control (control media alone). |
