| Cells were treated with different concentrations of SAM (0, 0.1, 1 and 10 μM)
for 24 h, and then with 1 mM H2O2 for 6 h. IGF-1 and Nrf2 mRNA expression
was measured using real-time PCR (B, C). IGF-1 and Nrf2 proteins were
measured using immunoprecipitation and Western blotting (D, E). The mean
intensity was measured using densitometry. SAM reversed the H2O2-induced
decreases in IGF-I and Nrf2 mRNA and protein levels (B, C, D and E), as well
as the decrease in cell viability (A). All expression levels are presented as the
mean ± SD of the fold- and percent-increases over that of the control (n=8).
Statistical analysis; *p<0.05, **p<0.01 vs. negative control (control media
alone). #p<0.05, ##p<0.01 vs. positive control (H2O2 alone). |
