| Cells were transfected with specific siRNA and then treated with SAM for
24 h. The cells were post-incubated with 1 mM H2O2 for 6 h. The effects of
IGF-I and Nrf2 siRNA on SAM-induced cell viability in the presence of H2O2
were measured with an MTT assay (A). All expression levels are presented
as the percent increase over the control. ROS generation was measured
using DCFH-DA (B). The fluorescent intensity was measured at 530 nm
after excitation at 490 nm. Fluorescent levels are expressed as the percent
increase over the control, and are expressed as mean ± SD (n=8). Statistical
analysis; *p<0.01, and **p < 0.05 vs. control media. # p<0.05 and ## p<0.01 vs.
media with H2O2 alone. & p < 0.05 vs. media with SAM and H2O2. |
