| Cells were transfected by specific siRNA and then treated with SAM for 24 h.
The cells were post-incubated with 1 mM H2O2 for 6h. The effects of IGF-I and
Nrf2 siRNA on SAM-induced endogenous IGF-I and Nrf2 in the presence of
H2O2 was measured using real-time PCR, and IGF-1 and Nrf2 protein levels
were measured using immunoprecipitation and Western blotting (A, B, C and
D). The mean intensity was measured using densitometry. The results are
expressed as mean ± SD of the fold-increases over that of the control (n=8).
Statistical analysis: *p<0.01, and **p<0.05 vs. control media. # p<0.05 and ##
p<0.01 vs. media with H2O2 alone. and p<0.05 and $$ p<0.01 vs. media with
SAM and H2O2. |
