| Cells were transfected by Nrf2 siRNA and then treated with exogenous IGF-I
for 24 h. The cells were post-treated with 1 mM H2O2 for 6 h. The effects
of endogenous IGF-I and Nrf2 on exogenous IGF-I and Nrf2 siRNA in the
presence of H2O2 was measured using real-time PCR, and IGF-1 and Nrf2
protein levels were measured using immunoprecipitation and Western blotting
(A, B, C and D). The mean intensity was measured using densitometry. The
results are expressed as the mean ± SD of fold-increases over that of the
control (n=8). Statistical analysis: *p<0.01, and **p<0.05 vs. control media. #
p<0.05 and ## p<0.01 vs. media with IGF-I and H2O2. & p<0.05 vs. media with
IGF-I and H2O2. |
