Figure 4: In situ reprogramming of spermine-treated hADMPCs into cardiomyocytes. (A) Photomicrographs of representative myocardial sections of the scarred area stained with hematoxylin/eosin (HE), Masson trichrome (MT) and Sirius red (SR) in spermine-treated hADMPC-transplantation and mock-transplanted control groups. Transplantation of spermine-treated hADMPCs resulted in the development of new cardiac muscles on the surface. Bars=300 μm. HE; hematoxylin and eosin staining, MT; Masson trichrome staining, and SR; Sirius red staining. (B) Spermine-treated hADMPC showed reprogramming In situ at 12 weeks after transplantation. Note the presence of human α-CA-positive (a and c) and human actinin-positive (b and d) cardiac muscle bundles or cells.