| Cell Types |
Methods of detection |
Paused percentage |
Categories of paused genes |
Pluripotency relation |
Reference |
| Mouse ESCs cultured with serum |
GRO-seq |
39% |
Pluripotency, DNA damage response, translation, cell cycle, and catabolism |
Pol II is not significantly paused at developmental regulators. Pluripotency genes are partially paused. |
[10] |
| MEFs |
GRO-seq |
34% |
DNA damage response, translation, cell cycle, and catabolism |
Not Discussed |
[10] |
| Mouse ESCs cultured with 2i |
GRO-seq and ChIP-seq of Pol II |
Not discussed |
Cell cycle, signal transduction, and cell proliferation |
Pol II is not significantly paused at developmental regulators. |
[14] |
| Mouse ESCs cultured with 2i |
ChIP-seq of Pol II |
Not discussed |
Not Discussed |
A general increase in PPP after growth in cells cultured with 2i than with serum. |
[51] |
| Mouse iPSCs |
ChIP-seq of Pol II |
Not discussed |
Oct4 in the later stage of reprogramming |
Klf4 recruits Cdk9 to the pluripotency promoters. |
[52] |
| Human ESCs cultured with serum |
ChIP-chip of Pol II-S5P |
52% |
Not discussed |
Not discussed |
[8] |
| Human hepatocytes |
ChIP-chip of Pol II |
36% |
Not discussed |
Not discussed |
[8] |
| Human B cells |
ChIP-chip of Pol II |
42% |
Not discussed |
Not discussed |
[8] |
