| Most neuron-glial antigen 2+ (NG2+) cells were pericytes (PCs) near Cluster of differentiation (CD) 31+ endothelial cells (ECs) at the embryonic (Embryonic Day 17
[E17]) and early postnatal (Postnatal Day 1 [P1]) stages. However, NG2+ PCs gradually decreased postnatally (P8), and were rare during adulthood (8 and 24 weeks).
NG2 (A: green); CD31 (A: red); 4’,6-diamidino-2-phenylindole (DAPI; A: blue; arrows). Similarly, Alpha smooth muscle actin (αSMA) was present in PCs near CD31+
ECs in embryonic (E17) and postnatal (P1 and P8) brains, but was rare during adulthood (8 and 24 weeks). αSMA (B: green); CD31 (B: red); DAPI (B: blue; arrows).
The numbers of NG2+ and αSMA+ PCs at each developmental stage are shown (C, D). Scale bars = 50 μm (A, B). |
