| In adult mice following ischemic stroke (A), Nestin was specifically induced in pericytes (PCs) near Cluster of differentiation (CD) 31+ cells within ischemic areas.
Nestin (B, C: green); CD31 (B, C: red); 4’,6-diamidino-2-phenylindole (DAPI; B, C: blue; arrows). In contrast, within contralateral non-ischemic areas (D), Nestin+ cells
were rarely PCs. Nestin (E: green); CD31 (E: red); DAPI (E: blue). At 8 and 24 weeks during adulthood, the number of Nestin+ PCs was significantly increased within
ischemic areas compared with in contralateral non-ischemic areas (F). It was confirmed that most Nestin+ cells within ischemic areas expressed the pericytic marker
platelet-derived growth factor receptor beta (PDGFRβ). Nestin (G, H, I: green); PDGFRβ (G, H, J: red); DAPI (G-J: blue). *p<0.05 versus non-ischemic areas at each
week (n = 3; F). Scale bars = 100 μm (B, G) and 20 μm (C, E, H). |
