Genes / OST Day 1 Day 7 Day 14 L Mean ±SD C Mean ±SD p value L Mean ±SD C Mean ±SD p Value LMean ±SD CMean ±SD p value GAPDH 0.00 ±0.00 0.00 ±0.00 0.8 0.00 ±0.00 0.00 ±0.00 0.9 0.00 ±0.00 0.00±0.00 0.9 NST 1.23 ±0.16 1.32 ±0.19 0.6 0.39 ±0.42 0.66 ±0.57 0.9 0.95 ±0.10 0.33 ±0.05 0.9 OCN 0.79 ±0.19 0.87 ±0.12 0.7 3.33 ±3.54 1.18 ±1.39 0.08 0.62 ±0.29 0.22 ±0.07 0.9 OPN 0.43 ±0.21 0.57 ±0.42 0.4 0.54 ±0.40 0.56 ±0.65 0.9 21.84 ±15.64 10.25 ±3.61 0.001 PCNA 0.87 ±0.15 0.91 ±0.04 0.4 2.76 ±2.01 2.92 ±3.62 0.9 0.98 ±0.10 0.51 ±0.05 0.001

Table 4: Comparison of Q-PCR expression of cell genes nucleostemin, osteocalcin, osteopontin, and PCNA after their equalization to the endogenous control gene (GAPDH) treated with LIPUS (L) 10 min/ day on days 1, 7, and 14: difference between LIPUS (L) and Control (C) in Osteogenic Media (OST). The results expressed as Mean ±SD.