(A) Schematic representation of stepwise reprogramming scheme. Human adult fibroblasts F134 were infected with SF-OSKM-tdTomato lentivirus shown in left panel. Four days post-infection, cells were re-plated and infected with Brg1 and Baf155 viruses. Four weeks after reinfection, human stem cell-like colonies were counted. (B) Schematic representation of simultaneous reprogramming scheme. Human adult fibroblasts F134 were infected with SF-OSKM-tdTomato along with Brg1 and Baf155 simultaneously. Two examples of reprogramming have been shown in left panel. Four weeks after infection, human stem cell-like colonies were counted. Data represent mean ± SEM of three virus-mediated transductions (n=3).

Figure 1: Induction of hiPSCs using OSKM and Brg1 and Baf155.