| (A) Transgene silencing was observed using red fluorescent protein expression four weeks after induction of pluripotency in OSKM and OSKMBB induced reprogramming. Absence of red fluorescent expression (middle panel) in colonies with human ESCs-like morphology (left panel) demonstrates transgene silencing. These colonies also show concomitant expression of alkaline phosphatase (right panel) analyzed by live staining. (B) Live analysis of OSKM and OSKMBB hiPSC colonies during reprogramming using Tra1-81 and CDy1 show similar expression level in both types of hiPSCs. |
