(A) Immunocytochemical analysis of pluripotency and surface markers (Alkaline phosphatase, TRA-1-60 and TRA-1-81, SSEA4, Nanog, OCT4,) in OSKMBB human iPSCs. (B) Immunofluorescence analysis of OSKMBB human iPSCs derived cells of all three germ layers after EB differentiation: ectoderm (β-tubulin lllb; TUJ1, mesoderm (α-smooth muscle actin; α-SMA) and endoderm (α-fetoprotein; AFP).

Figure 3: Characterization of OSKMBB hiPSCs.