| ALDH isozyme |
UCB ALDHbr Mean ΔCt (SD)a |
BM ALDHbr Mean ΔCt (SD)a |
BM ALDHdim Mean ΔCt (SD)a |
BM Fold regulationb |
Compartmentc |
| 1A1 |
3.36 (0.63) |
1.90 (0.73) |
8.32 (0.65)# |
102.96 |
Cytoplasmic |
| 1B1 |
10.00 (0.55)* |
7.40 ( 0.79) |
11.28 (0.66)# |
13.35 |
Mitochondrial |
| 4A1 |
7.15 (0.49)* |
4.79 (0.70) |
8.07 (1.01)# |
10.38 |
Mitochondrial |
| 5A1 |
6.75 (0.16) |
5.48 ( 0.71) |
8.28 (0.90)# |
8.11 |
Mitochondrial |
| 18A1 |
5.16 (0.41) |
4.07 (0.61) |
7.08 (0.66)# |
7.51 |
Mitochondrial |
| 6A1 |
5.68 (0.42) |
5.07 (0.62) |
7.04 (0.86)# |
4.37 |
Mitochondrial |
| 9A1 |
7.36 (0.34)* |
5.47 (0.68) |
7.46 (0.91)# |
4.27 |
Cytoplasmic |
| 16A1 |
6.73 (0.40) |
5.53 (0.78) |
7.63 (0.55)# |
4.26 |
Unknown |
| 7A1 |
7.10 (0.52) |
7.22 (0.25) |
8.54 (0.99)# |
2.56 |
Cytoplasmic |
| 3A2 |
7.76 (0.25) |
7.22 (0.61) |
7.95 (0.48) |
1.5 |
Microsomal |
| 1A3 |
12.84 (1.54) |
12.38 (1.23) |
12.37 (1.01) |
-1.24 |
Cytoplasmic |
| 3B1 |
7.06 (1.13) |
6.20 (0.52) |
5.53 (0.83) |
-1.29 |
Cytoplasmic |
| 2 |
4.73 (0.53)* |
6.65 ( 0.54) |
5.00 (0.60)# |
-3.79 |
Mitochondrial |
| 1L1 |
13.92 (1.20) |
13.42 (0.92) |
13.20 (1.03) |
-1.18 |
Cytoplasmic |
| 8A1 |
13.70 (0.77) |
13.49 (0.75) |
13.12 (1.03) |
-1.37 |
Cytoplasmic |
| 1L2 |
12.92 (0.64) |
13.28 (1.06) |
11.84 (0.75) |
-1.44 |
Mitochondrial |
| 1A2 |
15.23 (1.33) |
13.60 (0.83) |
13.12 (1.03) |
-1.51 |
Cytoplasmic |
| 3B2 |
13.63 (0.84) |
13.60 (0.83) |
13.12 (1.03) |
-1.51 |
Cytoplasmic |
| 3A1 |
13.67 (1.93) |
13.34 (0.97) |
12.99 (1.00) |
-2.34 |
Cytoplasmic |
a N=10 for BM ALDHbr; n=5 for BM ALDHdim; N=4 for UCB ALDHbr.
b Positive fold-regulation, ALDHbr>ALDHdim; Negative, ALDHdim>ALDHbr.
c Subcellular localization according to reference 22
* p< 0.05 for UCB ALDHbr as compared to bone marrow ALDHbr; t-test
# p< 0.05 for bone marrow ALDHbr as compared to bone marrow ALDHdim; N=5 matched pairs, t-test |
