ALDHbr vs. Hypoxic ALDHbr in monoculture ALDHbr vs. Hypoxic ALDHbr with HUVEC in transwells Gene Fold Regulation p value Fold Regulation p value Genes significantly upregulated under both culture conditions CXCL5 16.0 <0.001 140.3 0.02 EFNA1 11.2 <0.01 7.2 0.04 CXCL6 11.0  0.01 11.6 0.03 TIMP3 10.0 <0.01 20.3 0.02 COL4A3 9.1 <0.01 15.6 0.02 PLAU 9.1 0.03 6.0 0.01 FGF1 6.7 <0.001 22.6 0.02 PLG 6.7 <0.001 24.3 0.02 LEP 6.4 <0.001 23.7 0.02 CCL11 5.8 <0.001 22.2 0.02 HAND2 5.6 <0.001 21.9 0.02 PGF 3.6 0.0376 18.5 0.01 Genes significantly regulated in hypoxic monoculture only IL1B 6.4 0.03     KDR 4.7 0.04     TIMP2 4.2 0.03     CXCL9 3.7 0.01     JAG1 3.3 0.01     S1PR1 3.1 0.04     ID1 1.8 0.02     COL18A1 -9.2 0.03     Genes significantly upregulated in hypoxic cultures with HUVEC only IFNA1     16.1 0.02 EFNA3     11.9 0.03 IL6     9.8 0.02 ANGPTL3     8.1 0.02 CCL2     7.7 0.04 EGF     7.4 0.04 THBS2     6.9 0.03 SPHK1     3.5 <0.01

Table 3: Expression of angiogenesis genes by freshly isolated ALDHbr cells and ALDHbr cells cultured in transwells under hypoxic conditions with and without HUVEC networks. Cultures were maintained as described in the text. Values are fold change in mean expression of genes in the angiogenic array that were significantly (p<0.05) upregulated (positive values) or down regulated (negative values). Gene expression was measured by QRT-PCR.