Figure 3: Cells treated with 300 ÁM H2O2 for 72 hrs were examined by the AnnCy3 immunofluorescent method. When cells are incubated with both AnnCy3 and 6-CFDA, living cells and necrotic cells are labeled with 6-CF (green) and AnnCy3 (red), respectively. Cells in the early stage of apoptosis will be labeled with both AnnCy3 (red) and 6-CF (green). Staining with both AnnCy3 (red) and 6-CF (green), which appeared as yellow fluorescence, was frequently observed in ES-D7 cells treated with 300 ÁM H2O2. ES-D0 treated with 300 ÁM H2O2 showed only living cells. This figure was published in reference [12], Copyright Informa Healthcare.