Figure 1: Transcriptional Regulation of NANOG in ESCs. NANOG gene structure is shown at the bottom with the revised 5’ extended NANOG showing novel exons discovered recently in NTERA-2 cells [1]. NANOG regulatory regions are shown with DNAseI hypersensitivity peaks in H1 and H7 human ESCs. Chromatin occupancy profiles for ESC transcription factors (OCT4, NANOG), components of the basal transcriptional machinery (POL2 and p300) are shown at the NANOG locus in ESCs. The active chromatin mark H3 lysine 4 trimethylation (H3K4me3) profile and transcriptional expression levels generated by sequencing of polyA RNA are also depicted.