![]() (B) Alignment of the upstream P2 promoter in mouse, cat, cow, marmoset and human showing that the NANOG P2 is moving closer to the TSS across evolutionary time. (C) A schematic of the plasmid showing the design used for the neomycin resistance assay. (D) Neomycin resistant colonies produced by the respective promoters are plotted. Control cells were mock-transfected and the Hoxb6 promoter was used as a negative control as it is not active in ESCs. |
Figure 3: Neomycin resistance assay reveals alternate NANOG promoters in ESCs. |