(A) NANOG gene structure is shown at the bottom. DNAseI HS peaks in H1 ESCs, human cardiomyocytes (HCM), CD14 monocytes and human pancreatic adenocarcinoma cells (HPAF) showing the active cis-regulation mark at the upstream P2 promoter persists in adult differentiated cells, whereas the HS mark observed in the ESCs at the proximal P1 promoter is extinguished in the adult cells. (B) A similar persistence of the HS peak at P2 is observed in mouse adult cells while the downstream P1 peak is extinguished.
Figure 6: NANOG promoter P2 sequence conservation and DNAseI HS at the NANOG locus in different tissues.