Usage Primers Used (5’-3’) Amplimer size Promoter -Reporter hsNgProm -2401-AttB1-F1: ggggACAAGTTTGTACAAAAAAGCAGGCTTAtctctcatgcctttacccaaa hsNgProm -1817-AttB1-F1: ggggACAAGTTTGTACAAAAAAGCAGGCTTaaaaatctaaagtcagatagctt cc hsNgProm -1788-AttB1-F1: ggggACAAGTTTGTACAAAAAAGCAGGCTTAcctcaactttattccaattgctt t hsNgProm -1737-AttB2-R1: ggggACCACTTTGTACAAGAAAGCTGGGTAggccgacttactacattcttcg hsNgProm -1702-AttB2-R1: ggggACCACTTTGTACAAGAAAGCTGGGTAgtgaaagaccaaagggaagg hsNgProm -258-AttB1-F1: ggggACAAGTTTGTACAAAAAAGCAGGCTTAtcccattcctgttgaaccat hsNgProm +34-AttB2-R1: ggggACCACTTTGTACAAGAAAGCTGGGTAaacgttaaaatcctggagtctct Hoxb6 Prom-AttB1-F1: ggggACAAGTTTGTACAAAAAAGCAGGCTTaggttgataggtttgtgcgc Hoxb6 Prom-AttB2-R1: ggggACCACTTTGTACAAGAAAGCTGGGTAccgggtttatgatttgttgtgt   qRTPCR NANOG F3: tgtgggcctgaagaaaactatc NANOG R3: gctgtcctgaataagcagatcc NANOG F1: tccctttggtctttcactcc NANOG R1: ctccctgtcccattgtgtct NANOG F2: tatgcaaagacccccttctg NANOG R2: gctctccaaagggcaggta GAPDH F1: tgggtgtgaaccatgagaagta GAPDH R1: gagtccttccacgataccaaag 111bp 55bp 58bp 125bp

Table 1: Primers used in the Study. For the following promoter primers the attB site sequences are shown in capital letters. Information for EMSA primers can be found in Figure 5.