Figure 1: In vitro differentiation of neurosphere derived cells. A) Expression of GFAP defines astrocytes with typical flattened cell morphology. B) Immunocytochemical staining of cells with ßIII-tubulin. C) Staining of a cell with the NG2 antibody outlines the cell processes and cell body of an oligodendrocyte. D) Numerous cells co-expressed GFAP (green) and ßIII-tubulin (red) reflecting an uncommitted cell lineage. Indirect immunofluorescence on cells in 7 day old cultures. Scale bar = 25 µm.