Figure 4: Micrometastasis formation in the ovarioles of host flies, after transplantation of overproliferating brain tissue of numb RNAi knockdown. Confocal images of ovarioles of corresponding w1118 transplanted host flies: (A) insc-Gal4 driver control, (B) insc-Gal4 driven numb RNAi and (C) wor-Gal4, ase-Gal80 driven numb RNAi. In (B and C) the presence of GFP-labeled cells (in green) in the ovarioles indicates the presence of metastasis. DNA is labeled with Toto-3 iodide (in blue) and muscle layers by phalloidin (in red). Scale bars are 30 µm.