Figure 3: The SSEA-4 positive cells of human testis are enriched in SSCs. qRT-PCR analysis of human testicular cell populations isolated using MACS with antibodies to EpCAM, GPR125, SSEA-4, or ITGA6. ‘Testis’ is the unsorted testicular cell suspension. The bars represent the relative expression of EpCAM, GPR125, POU5F1, PLZF and SSEA-4 genes. GAPDH gene expression was also assayed for the normalization of the different RNA samples and all the reactions were performed in triplicates.