Figure 4: MMP2 does not play a key role in the tumor tropism of Human fetal MSC. (A) Western blot analysis was performed on 35μg of protein extracts from human adult MSC-1 and passage matched human fetal MSC S8, S27, S33 and S64 using anti-MMP2 antibody. Pan Actin was used as a loading control. Levels of total MMP2 were normalized to pan actin. (B) Expression of total secreted MMP2 in 100 μg of conditioned medium harvested from MSC-1, S8, S27, S33 and S64. Bar graph represents total MMP2 expression level normalized to MSC- 1. Data shown are averages of triplicate wells from representative experiments.