Figure 5: Migration of Human fetal MSC may involve Gα12/13 signaling pathway. (a) Western blot analysis on 35μg of protein extracts from human adult MSC-1 and passage matched human fetal MSC S8, S27, S33 and S64. Proteins were semi-quantified and normalized to loading control Pan Actin. Gα13 protein level were 0.01 (MSC-1), 0.03 (S8), 0.01 (S27), 0.22 (S33) and 0.01 (S64). p115RhoGEF protein levels were 0.02 (MSC-1), 0.09 (S8), 0.02 (S27), 0.04 (S33) and 0.00 (S64). Total Rho A protein levels were 0.45 (MSC-1), 0.33 (S8), 0.22 (S27), 0.13 (S33), 0.09 (S64). Total cdc42 protein levels were 0.17 (MSC- 1), 0.19 (S8), 0.14 (S27), 0.1 (S33), 0.05 (S64). Total Rac protein levels were 0.04 (MSC-1), 0.05 (S8), 0.03 (S27), 0.03 (S33) and 0.01 (S64). |