Figure 4: Expression of Oct-4, Suz-12 and Cripto-1 genes in PC3 (A) and LNCaP (B) prostate tumor cell. RNA was isolated and subjected to RT-PCR analysis; the amplified gene products were quantified by densitometric analysis and normalized to β-actin. (Student’s t-test, p<0.05). Plastic (2D cell cultures); 2-4-6d, 2-4-6 days of 3D cell cultures. |