Figure 5: Expression of Oct-4, Suz-12 and Cripto-1 protein in LNCaP (A) and PC3 (B) prostate tumor cells. Both cells grown directly on plastic slides (2D) and cytospins originated from cell spheroids (3D) were stained for the selected markers using ICA as described in materials and methods. Values represent average % of positively stained cells from 2 independent experiments performed in triplicate.