A) Control cells were Trypan blue negative after 3 and 6 hours of culture.
B) P. aeruginosa PA01 infected cells at 3 and 6 hours.
C) S. aureus Cowan I and
D) S .aureus KK1089 infected cells at 3 and 6 hours.
E) S. epidermidis ATCC 14990 and
F) S. epidermidis KK1087 infected cells at 3 and 6 hours. Magnification power was 200x in all panels. Red circles indicate Trypan blue positive nuclei. Red squares indicate changes in morphology. Black squares indicate the S. aureus produced aggregates.
Figure 2: Morphological changes induced in BMMSCs by bacterial infection. BMMSCs (line 412) were infected with bacteria, and after 3 and 6 hour incubation the cells were stained with Trypan blue and studied microscopically. Inoculum size of approximately 103 bacteria was selected for experiments.