Figure 5: Cardiac outgrowth-derived cells cultured in Mesencult for 2-3 months were transferred to cardiac or adipogenic differentiation media for 14 days. A. Immunostaining for sarcomeric a-actinin with Alexa 488 (green). Nuclei were stained with DAPI (blue). B. Immunostaining for a-SMA with Alexa 594 (red) and DAPI (blue). C. Merge. D/E. Phase photomicrographs of cardiac-derived cells showing adipogenic differentiation, as evidenced by intracytoplasmic fat droplets (low and high magnification views, respectively).