Figure 6: CS formation by cardiac outgrowth-derived cells from adult mice. A/B. Photomicrographs of CS (low and high magnification views, respectively). C. H&E staining of a CS section showing nuclei in the superficial layers as well as in the core. D. Immunostaining for tubulin A (green). E. Nuclear staining with Hoechst dye (nuclei). F. Merge. G. Effects of Mesencult medium supplementation with cytokines or growth factors on CS formation; DMEM-10% FCS was also tested for comparison (data are CS counts per well; *p < 0.05 vs. Mesencult).