Figure 7: Primary tissue culture from a neonatal Z/EG mouse heart. Upper left panel: Schematics of the Z/EG double reporter (lacZ, EGFP) system. Lower left panel: Tissue specificity of EGFP expression using AAV-9.Ncx1-CRE as compared with AAV-9.CMV-CRE. EGFP expression using the first vector was restricted to cardiomyocytes. Middle panel: Photomicrograph of Z/EG heart tissue explant and cell outgrowth. Right panel: Corresponding fluorescence photomicrograph (the boundaries of the tissue explant are indicated by a dashed line). Green fluorescence is detectable within the explant but not in the outgrowth.