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Table 1: Vector integration profile in LV1 clone upon multiple infections. The analysis was performed after genomic DNA digestions with NcoI+MseI or MseI restriction enzymes. The integration profile of LV1 has been acquired by LM-PCR and depicted a typical polyclonal integration pattern, as showed by multiple integration sites in open chromatin regions. Insertion sites detected outside genes are shown in bold, and the closest gene identified is reported in the table. |