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Table 2: Identification of vector insertions in transduced GFP+ Sgcb-null cardiac progenitors, and in derived clones. The analysis was performed on the genomic DNA by LM-PCR, after digestions with NcoI+MseI or MseI restriction enzymes. The overall distribution of the vector integration sites in the GFP+ sorted population confirmed the polyclonal profile of lentiviral integrations, while in the GFP+ clones (A8, C12, D1,F5 and G8), obtained by limiting dilutions, only one or two unique insertions were retrieved. Insertion sites detected outside genes are shown in bold, and the closest gene identified is also reported in bold in the table. |