Figure 1: Directed differentiation of the human bone marrow derived autologous pluripotent induced stem cells into endothelial cells. Gene expression products for and assembling of tight junctions: claudin 5 and zona occludens 1 (ZO-1) and adherens junctions: vascular endothelium cadherin and catenin prove efficacy of directed differentiation. Claudin and VE cadherin were highlighted by fluorescent red and cyan synthetic nano-antibodies. ZO-1 and catenin were highlighted by transgenic expression of their fusions with blue and yellow mutants of fluorescent proteins.