Figure 2: Human bone marrow and pluripotent induced stem cells directed to die. Human bone marrow mononuclear cells were induced into human autologous pluripotent stem cells (hapiSCs), which displayed cell surface biomarker SSEA-4, and selected with superparamagnetic synthetic nano-antibodies by MACS. After rapid cryoimmobilization, the cells were imaged with field emission scanning electron microscopy. The cells have round geometry and classical cell surface architecture (A). They vigorously proliferate, what is reflected in their geometry and cell surface architecture (B). The cells were transfected with the SSEA-4 antibody guided vectors coding DNASE1, DNASE1L3, DNASE2, and DFFB controlled by POLA promoter. Early signs of these transgenes’ expression, concurrent with G1/S transition, were blebs altering cell surface architecture of the hapiSCs (C). Some of these membrane blebs were developing advanced porosity – open routes for molecular entries (D).