Figure 5: Collapse of the ultrafine architecture of chromatin as the result of transgenic expression of the hrDNases. Human autologous pluripotent induced stem cells (hapiSCs) were directed to differentiate, while transfected with the SSEA-4 antibody guided vectors carrying coding sequences for DNASE1, DNASE1L3, DNASE2, and DFFB controlled by POLA1 promoter. The vectors delivered the transgenes for the hrDNases’only into the SSEA-4 displaying cells. Expression of these hrDNases occurred only in undifferentiated and proliferating stem cells, but did not occur in the differentiated cells. After rapid cryoimmobilization, the cells were imaged with field emission energy filtering transmission electron microscopy.