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Figure 6: Statistical evaluation of directed suicide in proliferating stem cell. A. The hapiSCs treated with the vectors for the hrDNases (Vector for hrDNases), with the vectors carrying the ORF in the reversed orientation (Vector for rev ORF), or with physiological buffer containing no vectors (No vector). At different time intervals, the cells were labeled with the superparamagnetic synthetic nano-antibodies targeting dsDNA. The results were quantified by measuring changes relaxivities detected in NMRS. First signs of directed stem cells’ death were already detected within the first hour since transfection. B. The hapiSCs (hapiSCs), human embryonic stem cells (heSCs), endothelial cells generated by induced directed vasculogenesis (idvECs), and human artery endothelial cells were treated the vectors for the hrDNases. At different time intervals, the cells were labeled with the element tagged anti-dsDNA antibodies and the scintillation were counted in EDXS. Proliferating hapiSCs and huECs were directed to die. Differentiated endothelial cells remained unaffected. |