Figure 2: In vitro assembly of MjK2 and temperature-dependent dissipation. A) Coomassie-stained (CO) 10% SDS-PAGE gel of the thermal denaturation experiment of the MjK2 channel protein obtained after enterokinase treatment at different temperatures (40-99.9°C) and intensity plots (B) quantified by densitometric scans of the corresponding gel lanes. Open circles describe the oligomer-to-monomer transition, which can be fitted by a sigmoid curve with a mid-point at ~92°C. Closed circles represent the monomer-to-tetramer transition with a midpoint at ~83°C.