Figure 10: pH-dependent zeta potential data for DNA and all polyions used in the present study is shown pertaining to measurements performed at 20 °C. Note the strong polyanionic nature of DNA, weak polycationic property of chitosan and polyampholytic behaviour of gelatin A and B, and BSA. b) Plot of solution turbidity measured at 450 nm as function of pH. At pHc associative interactions ensued and soluble complexes were formed marked A, (ii) at pHΦ soluble complexes coalesced to give rise to liquid-liquid phase separation, marked B and (iii) at pHprep formation of large insoluble complexes drove the solution to liquid-solid phase separation, marked C. Here, DNA concentration was maintained at 0.05% for GA and 0.5% for others. While polyion concentration used are 0.1% for GA, GB and BSA and 0.05% for chitosan. All measurements were performed at room temperature 20°C. [95] - Reproduced by permission of the PCCP Owner Societies.