Figure 2 Test to assess the bias of ThT test due to the presence of polyphenols. Fluorescence spectrum of ThT bound to κ-casein at the end of the aggregation process (black solid line) compared to the same spectrum registered upon adding 120 μg/ml of polyphenols. Spectra were then registered in time until 30 minutes from the polyphenols’ addition (red lines; from the upper to the lower, spectra are recorded every 5 minutes).