| Bile acid |
1st additiona
h |
2ndadditionb
h |
3rd additionc
h |
HADD
(Yield %) |
HADD
(Overall yield %)f |
| 1a |
24 |
24 |
32 |
2a (95)d |
2a (73) |
| 1b |
24 |
48 |
72-96 |
2b (23)d |
2b(18) |
| 1c |
24 |
24-32 |
72 |
2c (52)d |
2c (40) |
| 1d |
8-9 day |
-- |
-- |
2d (83)e |
|
|
| aTime of biotransformation with 1 g/L of bile acids (1a-d) as sodium salt; bTime
of biotransformation with 2 g/L of bile acids (1a-c) as sodium salt; cTime of
biotransformation with 10 g/L of bile acids (1a-c) as sodium salt; dCalculated on 10
g/L addition; eCalculated on 1 g/L addition; fCalculated on 13 g/L addition. |
| Table 1: Biotransformation of bile acids 1a-d with Pseudomonas alcaliphila. |
