Figure 2: Light microscopy of live preparations. Dynamic formation of spicules in vitro in cell culture of the L. baikalensis primmorphs: (A) primmorphs cultivated for three months in NBW; (B) primmorphs cultivated for three months in ABW in the absence of silicate; (C) primmorphs cultivated for three months in ABW in the presence of 70 µ silicate; (D) newly formed spicule on 2 day in NBW; (E) formation of spicule on 2 day in ABW; (F) formation of spicule on 2 day in ABW in the presence of 70 µ silicate; (G) formation of spicule on 12 day in NBW; (H) formation of spicule on 12 day in ABW; (I) formation of spicule on 12 day in ABW in the presence of 70 µ silicate. Scale bars: (A C) 0,5 mm; (D I) 10 .