Figure 8: SEM image of spicules in primmorphs of L. baikalensis cultivated in ABW in the presence of 70 µ silicate during three months. XMRA spectra obtained for freshwater spicules: (A) points spectra; (B) silica contents; (C) oxygen contents; (D) carbonate contents analysed the wall of a spicules. (E) the general silica contents of the spicules that were cultivated in ABW in presence of 70 µ silicate, as indicated on the spectra as percentages; (F) as an example, we provide spectrum 7, in which the silica content was 43.5%. Scale bars: 20 µ.