Figure 1: Illustration of the production, multiplication and plant regeneration from embryogenic cells in coffee (Coffea arabica). A) Formation of a primary callus constituted by meristematic cells at the edge of leaf explants 3 weeks following in vitro introduction; B) Embryogenic callus obtained 6 months after in vitro introduction; C) Somatic embryos regeneration from the embryogenic callus; D) Proliferation of embryogenic cell suspensions and subsequent somatic embryo regeneration in Erlenmeyer flasks agitated on orbital shakers in an industrial coffee micropropagation laboratory (CIRAD/ECOM unit, Sebaco, Nicaragua); E) Histological appearance of embryogenic cell clusters in suspension cultures; note that embryogenic cells exhibit a high nucleus cytoplasm ratio and dense cytoplasm rich in soluble and reserve proteins; F) Mass regeneration of torpedo-stage somatic embryos from embryogenic cell suspensions; G) Coffee somatic embryogenesis-derived plants in nursery (Cartago, Costa Rica); H) ‘Dwarf’ phenotypic variant (on the right) compared to a normal plant on the left; I) ‘Variegata’ phenotypic variant.