Figure 4: Effects of TJ-41 on tjhe upregulation of IL-1RI. Celles were treated with IL-1β (1nM) in the presence or absence of TJ-41 (6 mg/ml) for the indicated times. A: Phosphorylation of Akt. Total cell lysates were immunoprecitated with an anti-Akt antibody. B: Total RNA was analyzed by strand-specific RT-PCR to detect IL-1R1 mRNA, using EF mRNA as an internal control. C: Cell lysates (50µg of protein) were subjected tyo SDS-PAGE in a 7.5% gel, and immunoblotted with an anti-IL-1RI or anti - β-tubullin antibody.