Figure 6: Effect of TJ-41 on the expression of the iNOS gene antisense-transcript. Cells were treated with IL-1β (1 nM) in the presence or absence of TJ-41 (6 mg/ml) for the indicated times. Total RNA was analyzed by strand-specific RT-PCR to detect the iNOS gene antisense-transcript (iNOS-AST). RT(-): a negative control PCR using total RNA without RT.