HPLC System

 

LC 10

LC 20
Drugs Columns Conc. (µg/mL) Conc.Found (µg/mL) Recovery (%) Conc. Found (µg/mL) Recovery (%)
CAP Purospher STAR 8 8 100 8.06 100.75
10 10 100 9.98 99.8
12 12 100 12 100
Supelco C18 8 8 100 7.9 98.75
10 9.9 99 10 100
12 12 100 12 100
CIM Purospher STAR 8 8.1 101.25 8.0 100
10 9.9 99 9.88 98.8
12 12 100 12 100
Supelco C18 8 8 100 8 100
10 10 100 10.1 101
12 12 100 12.1 100.8
FAM Purospher STAR 8 7.9 99.7 8.03 100.3
10 9.99 99.9 9.98 99.8
12 12 100 12 100
Supelco C18 8 7.99 99.8 8 100
10 10 100 10 100
12 12 100 11.9 99.1
RAN Purospher STAR 8 7.98 99.7 8 100
10 9.99 99.9 9.9 99
12 12 100 11.9 99.1
Supelco C18 8 7.9 98.7 8.0 100
10 10 100 10.9 109
12 12 100 12.2 101.6
Table 5: Ruggedness of the method.