Drugs Conc (μg mL−1) Intraday precision Interday precision
% Rec % RSD % Rec % RSD
Phase-1
Lef 0.625 99.88 0.168 99.57 0.102
1.25 100.12 0.102 99.54 0.103
1.875 99.85 1.15 99.9 0.85
2.5 100.05 1.06 100.11 1.45
5 99.19 1.14 99.66 0.74
Flur 0.625 100.26 0.39 100.29 0.58
1.25 100.07 0.53 100.04 0.39
1.875 99.04 0.087 99.98 1.02
2.5 100.01 0.192 99.94 0.042
5 99.61 0.48 100.2 1.08
Ibu 11.25 100.41 1.12 99.97 0.166
22.5 100.02 1.02 98.54 0.32
33.75 100.10 0.24 100.2 1.08
45 99.76 0.29 99.89 0.33
90 99.42 0.17 100.12 0.90
Phase-2
Lef 0.625 99.78 0.34 99.57 0.08
1.25 99.88 0.06 99.54 0.27
1.875 100.02 0.02 100.29 0.29
2.5 99.57 0.03 100.18 0.87
5 100.48 0.63 99.66 0.06
Diclo 1.56 99.46 1.13 100.16 0.18
6.25 100.21 0.26 99.87 0.29
12.5 100.5 0.15 99.98 1.17
25 98.78 0.66 100.2 0.68
50 100.1 0.63 99.85 1.27
Mef 0.78 98.78 0.28 100.1 0.015
3.125 100.03 0.86 100.54 0.97
6.25 100.18 1.35 98.98 0.016
12.5 99.78 0.37 99.48 0.99
25 98.48 0.14 100.2 0.43
Table 2: Precision and recovery of leflunomide and NSAIDs.