High pool Low pool Methods Within-run CV% Between-day CV% Within-run CV% Between-day CV% TINIA 0.7 0.6 1.6 1.6 Cation-exchange HPLC 1.8 2.0 1.1 1.8 Affinity chromatography 0.4 0.1 0.5 0.2 Capillary electrophoresis 0.8 0.8 1.1 0.3

Table 1: Within-run and between day coefficients of variation (CV%) of HbA1c levels measured by the four methods using whole blood sample pool at low (33. 3 mmol/mol, 5.2 ± 0.05%) and high level (102.2 mmol/mol, 11.5 ± 0.06%).